Human Optineurin (OPTN) ELISA Kit

SKU: orb779102

Description

The Human Optineurin (OPTN) ELISA Kit uses a sandwich enzyme immunoassay to quantify Optineurin (OPTN) in human tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.058 ng/mL.

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OPTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OPTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OPTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OPTN in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.058 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

NRP ELISA Kit, FIP2 ELISA Kit, GLC1E ELISA Kit, HIP7 ELISA Kit, HYPL ELISA Kit, TFIIIA-INTP ELISA Kit, Glaucoma 1,Open Angle,E(Adult-Onset) ELISA Kit, Huntingtin-interacting protein 7 ELISA Kit, Optic neuropathy-inducing protein ELISA Kit, E3-14.7K-interacting protein ELISA Kit

Quick Database Links

UniProt

Q96CV9