Human Mucin 7, Secreted (MUC7) ELISA Kit

SKU: orb776067

Description

The Human Mucin 7, Secreted (MUC7) ELISA Kit uses a sandwich enzyme immunoassay to quantify Mucin 7, Secreted (MUC7) in human serum, plasma, saliva, tissue homogenates and other biological fluids. The kit has a detection range of 0.32-20 ng/mL and a sensitivity of 0.123 ng/mL.

Research Area

Cancer Biology, Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MUC7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MUC7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MUC7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MUC7 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, saliva, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.123 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

MG2 ELISA Kit, Mucin 7,Salivary ELISA Kit, Apo-MG2 ELISA Kit, Salivary mucin-7 ELISA Kit

Quick Database Links

UniProt

Q8TAX7