Human Ghrelin (GHRL) ELISA Kit

SKU: orb777944

Description

The Human Ghrelin (GHRL) ELISA Kit is designed to quantify Ghrelin (GHRL) in human serum, plasma and other biological fluids. The kit has a detection range of 78.13-5000 pg/mL and a sensitivity of 22.1 pg/mL.

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GHRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GHRL in the samples is then determined by comparing the OD of the samples to the standard curve.

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Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 78.13-5000 pg/mL
• Sensitivity: 22.1 pg/mL
• Concentration: 5000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

MTLRP ELISA Kit, Preproghrelin ELISA Kit, Growth Hormone Secretagogue Receptor Ligand ELISA Kit, Appetite-regulating hormone ELISA Kit, Growth hormone-releasing peptide ELISA Kit, Motilin-related peptide ELISA Kit

Quick Database Links

Gene Symbol

GHRL

UniProt

Q9UBU6