Human Filamin A Alpha (FLNa) ELISA Kit

SKU: orb778380

Description

The Human Filamin A Alpha (FLNa) ELISA Kit uses a sandwich enzyme immunoassay to quantify Filamin A Alpha (FLNa) in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 1.88-120 ng/mL and a sensitivity of 0.62 ng/mL.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 120 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FLNa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FLNa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FLNa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FLNa in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.88-120 ng/mL
• Sensitivity: 0.62 ng/mL
• Concentration: 120 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

ABP280 ELISA Kit, FLN-A ELISA Kit, ABPX ELISA Kit, FLN ELISA Kit, FLN1 ELISA Kit, FMD ELISA Kit, MNS ELISA Kit, NHBP ELISA Kit, OPD ELISA Kit, OPD1 ELISA Kit, OPD2 ELISA Kit, Actin Binding Protein 280 ELISA Kit, Endothelial actin-binding protein ELISA Kit, Filamin-1 ELISA Kit, Non-muscle filamin ELISA Kit

Quick Database Links

UniProt

P21333