HighYield T7 STAR RED RNA Labeling Kit (UTP-based)

SKU: orb1733301

Description

HighYield T7 STAR RED RNA Labeling Kit (UTP-based) is designed to produce randomly abberior STAR RED-modified RNA probes viain vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. abberior STAR RED is a hydrophilic dye with remarkable photostability that allows opti-mal performance of dye-conjugates in STED and confocal microscopy. UTP-PEG5-STAR RED is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35% UTP-PEG5-STAR RED substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of UTP-PEG5-STAR RED/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting abberior STAR RED-modified RNA probe can subsequently be detected by fluorescence spectroscopy. The kit contains sufficient reagents for 20 labeling reactions of 20 µl each (35% UTP-PEG5-STAR RED substitution, 2.5 mM ATP, GTP, CTP, 0.2 mM UTP, 0.1 mM UTP-PEG5-STAR RED).

Research Area

Cell Biology

Images & Validation

• Application Notes:
  Spectroscopic Propertie: λexc 638 nm, λem 655 nm, ε 120.0 L mmol-1, cm-1 (Tris-HCl pH 7.5).

Key Properties

• SMILES: P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H]([C@@H]([C@@H]1O)O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCOCCOCCOCCOCCOCC[NH][C]

Storage & Handling

• Storage: store at -20 °C. avoid freeze/thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Preparation of randomly abberior STAR RED-modified RNA probes by in vitro transcription with UTP-PEG5-STAR RED

Compound Identifiers

PubChem CID

176418718