Guanfacine hydrochloride

SKU: orb1306327

Description

Guanfacine hydrochloride

Research Area

Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 29110-48-3
• MW: 282.55
• Purity: 99.91% (May vary between batches)
• Formula: C₉H₁₀Cl₃N₃O
• SMILES: Cl.NC(=N)NC(=O)Cc1c(Cl)cccc1Cl
• Target: Adrenergic Receptor
• Solubility: H2O:28.3 mg/mL (100.16 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.08 mM);DMSO:55 mg/mL (194.66 mM)

Bioactivity

• Target IC50:
  α2B-adrenoceptor:31 nM(Kd)
• Cell Research:
  Cells (1 mL) are seeded into 24-well plates at a density of 1 × 105 cells/mL. Culture medium is replaced every two days until cells reached confluence. To investigate the potential influence of P-gp on guanfacine transport, intracellular accumulation of guanfacine is measured in LLC-PK1/MDR1 cells and P-gp-negative LLC-PK1 cells. Rhodamine6 g, a well-known P-gp substrate, is included as a positive control. After cells reach confluence, the culture medium is replaced by transport buffer for a 30-minute preincubation at 37°C. Transport buffer is then removed and the test compounds (5 μM rhodamine6 g, 5 μM guanfacine, or 50 μM guanfacine) are added, and an additional 60-minute incubation period commenced. Next, the solutions are discarded, and the cells are washed three times with ice-cold Dulbecco's phosphate-buffered saline and solubilized with 1% Triton X-100. Intracellular accumulation of rhodamine6 g is measured using a fluorescent microplate reader with a 530 nm excitation and a 580 nm emission wavelength, which is a well established method. Cellular retention of guanfacine is analyzed by the high-performance liquid chromatographic assay described below. The concentrations of rhodamine6 g and guanfacine are calculated from their respective standard curves, and standardized with the cellular protein content determined by a Pierce bicinchoninic acid protein kit.(Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

hypotension, noradrenergic α2A, Intuniv, Intuniv hcl, cognitive disorders, Adrenergic Receptor, AdrenergicReceptor, attention deficit hyperactivity disorder (ADHD), prefrontal cortex (PFC), sedation, tourette's syndrome, α2A-adrenoceptor, α2B-adrenergic receptor, α2A receptor, Tenex, Tenex hcl

Compound Identifiers

PubChem CID

71401