Green Fluorescent Cathepsin B Assay

SKU: orb1409901

Description

The quenched substrate Rhodamine 110-(RR)2, fluoresces green upon cleavage by active cathepsins, allowing the monitoring of intracellular cathepsin activity in vitro using live whole cells. Analyze using flow cytometry or fluorescence microscopy.

Images & Validation

• Application Notes:
  1. Dilute 10X Cellular Assay Buffer 1:10 with diH2O. 2. Prepare samples and controls in fresh cell culture medium or 1X Cellular Assay Buffer. 3. Reconstitute R110-(RR)2 with 50 µL DMSO. 4. Dilute R110-(RR)2 1:5 by adding 200 µL PBS. 5. Add diluted R110-(RR)2 to each sample at 1:50 (e.g. add 10 µL of diluted R110-(RR)2 to 490 µL of sample). 6. Incubate while protected from light. 7. If desired, label with additional stains, such as Hoechst 33342, DAPI, or an antibody. 8. Analyze with a flow cytometer or fluorescence microscope. R110-(RR)2 excites at 500 nm and emits at 525 nm.

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Key Properties

• Sample Types: Cell culture

Storage & Handling

• Storage: R110-(RR)2 at ≤-20°C, other components at 2-8°C
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only