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NBL4 Antibody

SKU: orb19725

Description

Goat polyclonal antibody to EPB41L4A

Images & Validation

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Tested ApplicationsELISA
Dilution RangeELISA: 1:8000, WB: 1 μg/ml
Predicted ReactivityHuman, Mouse
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 100kDa band in Human Brain (Cerebellum) lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.0kDa according to NP_071423.3. The 100kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

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ClonalityPolyclonal
TargetNBL4
Protein SequenceKNENSPDTQRSKSH
Molecular Weight79
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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anti EPB41L4A antibody, anti erythrocyte membrane protein band 4.1 like 4A antibody, anti EPB41L4 antibody, anti FLJ38738 antibody, anti NBL4 antibody, anti erythrocyte protein band 4.1-like 4 antibody

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NCBI Gene Details

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No NCBI Gene data available

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 360.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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