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GGA1 Antibody

SKU: orb345491

Description

GGA-1 antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA, IP, WB
Dilution RangeELISA: 1:10,000 - 1:50,000, IP: 1:100, WB: 1:500 - 1:3,000
ReactivityHuman
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect bands at 70 kDa in size corresponding to GGA1 by western blotting in the appropriate cell lysate or extract.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenThis affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 330-360 of Human GGA-1.
TargetGGA1
PurityThis affinity purified antibody is directed against human GGA1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. Cross reactivity may occur with GGA1 from bovine (87% homology), mouse (82% homology) and rat (76% homology) sources. The immunizing sequence is present only in isoform 1 of this protein, therefore cross reactivity with splice variants is not expected. Cross reactivity with GGA1 homologues from other sources has not been determined.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.35 mg/mL
Expiration Date12 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

rabbit anti-GGA-1 rabbit, GGA 1,GGA1, ADP-ribosylation factor binding protein, Golgi-localized gamma ear-containing ARF-binding protein 1, Gamma-adaptin related protein 1

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeq9558729

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

Available Sizes

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100 μg
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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