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General Lipopolysaccharides (LPS) ELISA Kit

SKU: orb1946412

Description

General Lipopolysaccharides (LPS) ELISA Kit

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LPS. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to LPS. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LPS in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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ReactivityAll
Sample TypesSerum, Plasma, Tissue homogenate and Other biological samples
Assay TypeCompetitive
Assay Time1.5H
Range1.56-100 pg/mL
Sensitivity0.94 pg/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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Lipopolysaccharides

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