General 4-Hydroxynonenal (4-HNE) ELISA Kit

SKU: orb1146751

Description

The General 4-Hydroxynonenal (4-HNE) ELISA Kit is designed to quantify General 4-Hydroxynonenal (4-HNE) in serum, plasma, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 0.63-40 ng/mL and a sensitivity of 0.38 ng/mL.

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.

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Key Properties

• Reactivity: All
• Sample Types: serum, plasma, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.38 ng/mL
• Concentration: 40 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal

Quick Database Links

Gene Symbol

4-HNE