Ganoderenic acid B

SKU: orb1698431

Description

Ganoderenic acid B is a natural lanostane triterpenoid isolated from Ganoderma lucidum that acts as an ABCB1 transporter inhibitor. It reverses multidrug resistance in vitro by enhancing the cytotoxicity of chemotherapeutic agents in ABCB1-overexpressing cancer cells.

Research Area

Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 100665-41-6
• MW: 514.65
• Purity: 99.93% (May vary between batches)
• Formula: C₃₀H₄₂O₇
• SMILES: CC(CC(=O)\C=C(/C)[C@@H]1CC(=O)[C@@]2(C)C3=C(C(=O)C[C@]12C)[C@@]1(C)CC[C@H](O)C(C)(C)C1C[C@@H]3O)C(O)=O
• Target: P-gp
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (6.41 mM);DMSO:90 mg/mL (174.88 mM)

Bioactivity

• In Vitro:
  Ganoderenic acid B, a lanostane-type triterpene isolated from Ganoderma lucidum, exhibited potent reversal effect on ABCB1-mediated multidrug resistance of HepG2/ADM cells to doxorubicin, vincristine and paclitaxel. Similarly, Ganoderenic acid B could also significantly reverse the resistance of ABCB1-overexpressing MCF-7/ADR cells to doxorubicin. Furthermore, Ganoderenic acid B notably enhanced intracellular accumulation of rhodamine-123 in HepG2/ADM cells through inhibition of its efflux. ABCB1 siRNA interference assay indicated that the reversal activity of Ganoderenic acid B was dependent on ABCB1. Further mechanistic investigations found that Ganoderenic acid B did not alter the expression level of ABCB1 and the activity of ABCB1 ATPase. Molecular docking model displayed that the positions of Ganoderenic acid B binding to ABCB1 were different from the region of verapamil interacted with ABCB1. Ganoderenic acid B can enhance the cytotoxicity of chemotherapeutics towards ABCB1-mediated MDR cancer cells via inhibition of the transport function of ABCB1. These findings provide evidence that Ganoderenic acid B has the potential to be developed into an ABCB1-mediated multidrug resistance reversal agent.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ABCB1, Ganoderenic acid B, Pgp

Compound Identifiers

PubChem CID

164576414