FLICA 660 Poly Caspase Assay Kit

SKU: orb1409918

Description

This in vitro assay employs the far-red fluorescent inhibitor probe 660-VAD-FMK to label active caspase enzymes in living cells. Analyze the fluorescent signal using fluorescence microscopy or flow cytometry.

Research Area

Cell Biology

Images & Validation

• Application Notes:
  1. Prepare samples and controls. 2. Dilute 10X Cellular Wash Buffer 1:10 with diH20. 3. Reconstitute FLICA with 50 µL DMSO. 4. Dilute FLICA 1:5 by adding 200 µL PBS. 5. Add diluted FLICA to each sample at 1:30-1:60 (e.g. spike at 1:30 by adding 10 µL to 290 µL cultured cells). 6. Incubate approximately 1 hour. 7. Remove media and wash cells 3 times: add 1X Cellular Wash Buffer and spin cells. 8. If desired, label with additional stains, such as Hoechst, DAPI, or an antibody. 9. If desired, fix cells. 10. Analyze with a fluorescence microscope or flow cytometer. FLICA 660 excites at 660 nm and emits at 680-690 nm.

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Key Properties

• Sample Types: Cell culture

Storage & Handling

• Storage: 2-8°C
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only