FLICA 660 Caspase-1 Assay Kit

SKU: orb1409917

Description

This in vitro assay employs the far-red fluorescent inhibitor probe 660-YVAD-FMK to label active caspase-1 enzyme in living cells. Analyze samples using fluorescence microscopy, or flow cytometry.

Research Area

Cell Biology

Images & Validation

• Application Notes:
  1. Prepare samples and controls. 2. Dilute 10X Binding Buffer 1:10 with diH2O. 3. Wash cells in ice-cold culture medium or PBS and resuspend in ice-cold 1X Binding Buffer. 4. Dilute Annexin V-FITC 1:10 in PBS. 5. Dilute Propidium Iodide (PI) 1:2 in PBS. 6. Add diluted Annexin V-FITC to each sample at a ratio of 1:20 (5 µL per 100 µL aliquot of cultured cells). 7. Add diluted PI to each sample at a ratio of 1:20 (5 µL per 100 µL aliquot of cultured cells). 8. Incubate ~10 minutes, protected from light. 9. Dilute samples by adding 250 µL 1X Binding Buffer to each. 10. Analyze with a flow cytometer. Annexin V-FITC optimally excites at 494 nm and emits at 519 nm (typically read in FL1). PI optimally excites at 536 nm and has a peak emission at 617 nm (typically read in FL3).

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Key Properties

• Sample Types: Cell culture

Storage & Handling

• Storage: 2-8°C
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only