FAM-FLICA Caspase 10 Assay Kit

SKU: orb1409954

Description

This in vitro assay employs the green fluorescent inhibitor probe FAM-AEVD-FMK to label active caspase-10 enzyme in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.

Research Area

Cell Biology

Images & Validation

• Application Notes:
  1. Prepare samples. 2. Create controls with CCCP. 3. Dilute 10X Assay Buffer 1:10 with diH2O. 4. Reconstitute MitoPT JC-1 with DMSO. 5. Dilute MitoPT JC-1 with 1X Assay Buffer. 6. Add MitoPT JC-1 to each sample. 7. Incubate 15-30 minutes. 8. Wash cells: add 1X Assay Buffer and spin cells (twice). 9. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. MitoPT JC-1 excites at 488 nm. Aggregated MitoPT JC-1 (orange) emits at 590 nm, monomeric MitoPT JC-1 (green) emits at 527 nm.

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Key Properties

• Sample Types: Cell culture

Storage & Handling

• Storage: 2-8°C
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only