Edetic acid

SKU: orb1219778

Description

Edetic acid is a chelating agent commonly used in protein purification, both to eliminate contaminating divalent cations and to inhibit protease activity.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 60-00-4
• MW: 292.24
• Purity: >98% (HPLC)
• Formula: C₁₀H₁₆N₂O₈
• SMILES: OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• Target: Others
• Solubility: In Vitro: 0.1 M NaOH : 6.67 mg/mL (22.8)

Bioactivity

• In Vivo:
  Animal model: Male Wistar rat model of cirrhosis induced by CCl4. Dosage: 60 mg/kg, 120 mg/kg, 240 mg/kg. Administration: Intraperitoneal injection (i.p.); Three times per week for 3 weeks (during this period, CCl4 administration continued). After CCl4 and mineral oil mixture treatment (200 μL/mouse; i.p.; Three times per week for eight weeks). Result: Kept kept all the rats alive at the 60 mg/kg dose, but died at 120 and 240 mg/kg. Reduced fibrosis of the liver in surviving rats (20%). Animal model: Male Wistar rat model of cirrhosis induced by CCl. Dosage: 60 mg/kg. Administration: Intraperitoneal injection (i.p.); Three times per week for 11 weeks. EDTA and CCl4 were administered during 11 weeks, three times per week on alternate days). Intraperitoneal injection (i.p.); Three times per week. After CCl4 treatment (i.p.; Three times per week for eight weeks) (Therapeutic EDTA group: EDTA and CCl4 were administered for 3 weeks, three times per week on alternate days). Result: Increased sod activity by 50% in the preventive EDTA group. (Compared with untreated EDTA group)Increased Cp activity in the preventive EDTA (30%) and therapeutic EDTA (20%) groups. (Compared with the fibrotic group). Decreased mRNA expression of the pro-inflammatory molecule (TNF-α and LI-6) and the profibrogenic molecules (TGF-β and αCOLI) in both the prevention and treatment groups.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ethylenediaminetetraacetic acid,EDTA

Quick Database Links

Gene Symbol

Others