Digoxigenin

SKU: orb2901182

Description

Digoxigenin (DIG) is a steroid. DIG is used for situ hybridization asa labeling molecule probedue to long shelf life and fast detection and high sensitivity of DIG-labeled riboprobes.

Key Properties

• CAS Number: 1672-46-4
• MW: 390.51
• Purity: >98% (HPLC)
• Formula: C₂₃H₃₄O₅
• SMILES: O[C@@]12[C@@](C)([C@H](CC1)C=3COC(=O)C3)[C@H](O)C[C@]4([C@]2(CC[C@]5([C@]4(C)CC[C@H](O)C5)[H])[H])[H]
• Solubility: In Vitro: DMSO : 100 mg/mL (256.08 mM; Ultrasonic )

Bioactivity

• In Vitro:
  Cell Viability Assay Cell line: HepG2 (human hepatocellular carcinoma) cell line. Concentration: 0-10 μM. Incubation time: 24 h. Result: Identified the optimal concentration for Digoxigenin: 1 μM. Cell Cytotoxicity Assay Cell line: RORγ-HepG2 cells. Concentration: 0-2 μM. Incubation time: 24 h. Result: Increased the luciferase activity with concentration-dependent in cells.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only