Concanavalin A

SKU: orb1303318

Description

Concanavalin A is a plant lectin with glycan-binding properties, studied for its antitumor effects in vitro and in vivo. It is also a well-documented model agent for investigating immune-mediated acute liver injury in experimental research settings.

Research Area

Cell Biology, Infectious Disease & Virology

Key Properties

• CAS Number: 11028-71-0
• MW: 104-112 kDa
• Formula: C₂₃H₃₂N₆O₈S
• SMILES: CCCN(CCC)S(=O)(=O)c1ccc(cc1)C(O)=O.Cc1cn(C2CC(N=[N+]=[N-])C(CO)O2)c(=O)[nH]c1=O
• Target: Antibiotic,Apoptosis
• Solubility: H2O:soluble;PBS:5 mg/mL

Bioactivity

• Target IC50:
  ML-1 cells:10 μg/mL|CT-26 cells:10 μg/mL|HIV-1:2.2–2.6 μg/mL (EC50)|HSV:98 nM|HepG2 cells:5 μg/mL|MCF-7 cells:15 μg/mL|Huh7 cells:20 μg/mL
• In Vivo:
  METHODS: To assay antitumor activity in vivo, Concanavalin A (10 mg/kg, intravenously) and ISO-1 (10 mg/kg, intraperitonealy) were administered to BALB/c mice bearing ML-1 liver tumor nodules twice every three days for 30 days. RESULTS: Concanavalin A treatment significantly reduced the number and size of hepatic tumor nodules and, in the presence of ISO-1, the therapeutic effect of Concanavalin A was abolished. MIF does play an important role in Concanavalin A-induced autophagy and antitumor activity in vivo.
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• In Vitro:
  METHODS: Human hepatocellular carcinoma cells HuH-7 and Hep G2 were treated with Concanavalin A (1-40 µM) for 3-48 h, and the expression levels of target proteins were detected by Western Blot. RESULTS: After Concanavalin A stimulation, LC3-II in both human hepatocellular carcinoma cell lines increased in a dose- and time-dependent manner. METHODS: HUVECs and EA.hy926 cells were treated with Concanavalin A (0.3-1 µg/mL) for 6-24 h. The pro-angiogenic activity was detected by Matrigel tube formation and ELISA assay. RESULTS: Different concentrations of Concanavalin A, especially at 1 or 3 µg/mL, significantly promoted angiogenesis in HUVEC and Ea.hy926 cells. The total primary fragment length in HUVEC and Ea.hy926 cells was significantly higher in Concanavalin A-treated cells compared to the negative control.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Antibiotic, Apoptosis, Concanavalin A, jack bean, inhibit, glucose, Inhibitor, mannose, plant lectin, programmed cell death

Compound Identifiers

PubChem CID

155486958