Chalcone

SKU: orb1221956

Description

trans-Chalcone is an aromatic ketone that forms the central core for a variety of important biological compounds, which are known collectively as chalcones.

Key Properties

• CAS Number: 614-47-1
• MW: 208.26
• Purity: >98% (HPLC)
• Formula: C₁₅H₁₂O
• SMILES: c1ccc(cc1)/C=C/C(=O)c1ccccc1
• Target: p53
• Solubility: In Vitro: DMSO : 100 mg/mL (480.17 mM)

Bioactivity

• In Vitro:
  trans-Chalcone competitively inhibits porcine pancreatic α-amylase with a Ki of 48 μM. trans-Chalcone (30.23-98.03 μM; 24 hours) induces cell cycle arrest and apoptosis in MCF-7 cells. trans-Chalcone (20-80 μM; 24, 48 hours) reduces the expression of the apoptosis-related protein Bcl-2. trans-Chalcone (58.25 μM; 6, 24 hours) has greater inhibition of Bcl-2, induction of APAF1 and BAX, and strong induction of CIDEA in 24 hours. trans-Chalcone (24 hours) inhibits MCF-7 cell viability (IC20 = 30.23 μM; IC50 = 58.25 μM; IC80 = 98.03 μM). trans-Chalcone (48 h) has IC50s of 41.53 μM and 48.41 μM for MCF-7 and 3T3 cell lines, respectively. trans-Chalcone exhibits a pronounced cytotoxicity activity. Apoptosis Analysis Cell line: MCF-7 cell. Concentration: 30.23, 58.25, 98.03 μM Incubation time: 24 hours. Result: Induced apoptosis of the breast cancer cell line. Cell Cycle Analysis Cell line: MCF-7 cell. Concentration: 30.23, 58.25, 98.03 μM Incubation time: 24 hours. Result: Caused cell cycle arrest in G1. Western blot analysis. Cell line: MCF-7 cell. Concentration: 20, 40, 80 μM. Incubation time: 24, 48 hours. Result: Reduced the expression of the apoptosis-related protein Bcl-2 and induced the expression of the CIDEA gene. There was marked degradation of cyclin D1 at 48 h. RT-PCR Cell line: MCF-7 cell. Concentration: 58.25 μM Incubation time: 6, 24 hours. Result: Had greater inhibition of Bcl-2, induction of APAF1 and BAX, and strong induction of CIDEA in 24 hours.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

p53