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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
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| Tested Applications | FC, ICC, IP |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however as a guideline try the following: IP: Use at 3µg/mg of lysate. WB: Use a concentration of 0.5 - 1µg/ml. Predicted molecular weight: 312 kDa. Only suitable for WB if IP is performed first Flow Cyt: Use 1µg for 106 cells |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 kappa |
| Clone No. | 1H12 |
| Immunogen | Recombinant full length CENPE protein (human) |
| Target | CENPE |
| Purification | Affinity purified by ion exchange chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody (from supernatant) containing PBS 0.1% sodium azide; |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CENP E antibody;Centromere autoantigen E antibody;Centromere protein E 312kDa antibody;Centromereprotein E antibody;Centromeric protein E antibody;KIF10 antibody;Kinesin family member 10 antibody;Kinesinrelated protein antibody
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