CD40LG Antibody (Biotin)

SKU: orb751205

Description

Mouse monoclonal antibody against CD40LG conjugated to Biotin

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA (For coating, order antibody without BSA); Flow Cytometry (2-4ug/million cells); ,Immunofluorescence (2-4ug/ml); Western Blot (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: Jurkat cells. Tonsil, Spleen or Thymus

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: CD40LG/2761
• Immunogen: Recombinant fragment (around aa 108-261) of human CD40LG protein (exact sequence is proprietary)
• Target: CD40LG
• Source/Expression System: Bioreactor
• Molecular Weight: 36kDa
• Purification: Protein A
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-CD154 antibody, anti-gp39 antibody, anti-hCD40L antibody, anti-HIGM1 antibody, anti-IGM antibody, anti-IMD3 antibody, anti-T B cell activating molecule antibody, anti-T BAM antibody, anti-T-cell antigen Gp39 antibody, anti-TNF-related activation protein antibody, anti-TrAP antibody, anti-Tumor necrosis factor ligand superfamily member 5 (TNFSF5) antibody

Quick Database Links

Gene Symbol

CD40LG

UniProt

P29965

NCBI Gene ID

959