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Description
Research Area
Cell Biology, Cell Differentiation, Kinases, Signal Transduction, Tyrosine Kinases
Images & Validation
−| Tested Applications | ELISA, IF, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200, ELISA=1:30-3000, IP=1:100-500 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a, κ |
| Clone No. | B5I1 |
| Immunogen | Recombinant human B-Raf protein (12-156/766aa) |
| Target | BRAF |
| Molecular Weight | 95 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−B-RAF1; B-raf; BRAF-1; BRAF1; NS7; RAFB1; 9930012E13Rik; Braf-2; Braf2; C230098H17; D6Ertd631e; BRAF_HUMAN; BRAF; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; 2.7.11.1; BRAF_MOUSE; B-Raf proto-oncogene, serine/threonine kinase; v-raf murine sarcoma viral oncogene homolog B
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Quick Database Links
Gene Symbol
BRAF
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation






















