BiorbytNext ATAC-seq Library Prep Kit for Illumina

SKU: orb3229945

Description

This is an ATAC-seq library construction kit developed for Illumina's high-throughput sequencing platform for 103~105 Sample building for a starting volume of one cell.ATAC-seq (Assay for Transposase Accessible Chromatin with high-throughput sequencing) is a high-throughput sequencing technology mediated by transposase for rapid detection of open regions of chromatin on a genome-wide scale; it can reveal the association between chromatin accessibility and gene expression regulation, providing key clues for epigenetic studies; it is applicable to epigenetic studies such as chromosome openness mapping, embryonic development, prediction of potential markers of diseases, tumorigenesis, etc. This product uses Tn5 transposon containing part of illumina sequencing junction, combined with chromatin open region, to cut naked DNA and add sequencing junction at the same time, and then obtain sequencing library after PCR amplification, which has the advantages of wide range of cellular starting range, short experimental period, high signal-to-noise ratio, and wide range of applications. The reaction system of this product has been carefully optimized, all reagents in the experiment should be used as provided in this product, it is not recommended to change any reaction components or replace the components in this product with other equivalent products in order to avoid obtaining bad experimental results. If replacement is needed, please verify it first.

Images & Validation

• Application Notes:
  1. For illumina sequencing platform: integrates Index and illumina connectors and constructs DNA libraries that can be directly sequenced by NGS. 2. High throughput: ATAC-seq is capable of analyzing a large number of samples in a short period of time, which is suitable for large-scale genomic research. 3. Low Cell Requirement: ATAC-seq requires a lower number of cells than traditional methods and can be performed from as low as 1000 cells. 4. Wide range of cellular inputs: applicable for 103~105 Sample building for a starting volume of cells. 5. High Repeatability: The experimental results have a high degree of repeatability and consistency. 6. High signal-to-noise ratio: TSS is well enriched and has a high signal-to-noise ratio.

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Storage & Handling

• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only