AVS Bio - Human PAD4

SKU: MQ16.203

Description

Peptidylarginine deiminase 4 (PAD4) is an enzyme that catalyzes the conversion of arginine residues in proteins to citrulline, a process known as citrullination or deimination. This post-translational modification can alter protein structure, charge, and function, influencing processes such as gene regulation, chromatin remodeling, and immune responses. PAD4 has been linked to autoimmune diseases like rheumatoid arthritis and might be involved in neutrophil extracellular trap (NET) formation.

Images & Validation

• Application Notes:
  Recombinant human PAD4 is intended for studies on its deimination properties.

Key Properties

• Biological Activity: Activity has been tested in an MQR Antibody Based Assay for PAD activity (ABAP) (Cat. no. MQ-17.101) and compared to rabbit skeletal muscle PAD from Sigma-Aldrich (figure 1).
• Purification: Recombinant human PAD4 comes with a HIS-tag and has been purified from bacterial cell lysate using Ni 2+-beads.

Storage & Handling

• Storage: Store at - 80°C. *AVOID FREEZE THAW*
• Form/Appearance: solution
• Buffer/Preservatives: Solution in 20mM Tris-HCl, pH 7.6, containing 10mM β mercaptoethanol, 400mM NaCl, 250mM Imidazole, 1mM EDTA and 10% glycerol.
• Expiration Date: For shelf life and expiration details, please refer to the Certificate of Analysis (CoA).
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only