AsCas12a (Cpf1) Nuclease

SKU: orb3229968

Description

AsCas12a (cpf1) Nuclease is a nucleic acid endonuclease from Acidaminococcus sp. This product is a recombinant protein obtained by constructing the AsCas12a Nuclease gene sequence into a plasmid, recombinantly expressing and purifying it in E. coli. AsCas12a enzyme, guided by crRNA, specifically shears the target double-stranded DNA in the presence of PAM (TTTV, V=A/C/G) sequence and forms sticky ends. In addition, this product has trans-shearing activity, i.e., when AsCas12a enzyme binds to crRNA and target double-stranded DNA to form a ternary complex, it activates the trans-shearing activity against non-specific ssDNA sequences, and fragments any ssDNA sequences in the system.

Storage & Handling

• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only