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CD3 zeta (pY72) Antibody

SKU: orb179876

Description

Mouse monoclonal antibody to CD3 zeta

Research Area

Immunology & Inflammation

Images & Validation

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Tested ApplicationsFC, ICC, WB
ReactivityHuman, Mouse
Application Notes
Western blotting: Recommended dilution: 2 - 5 μg/ml; positive control: Jurkat cells lysate treated with pervanadate, splenocyte lysate of Balb/c or F1 mouse treated with pervanadate, non-reducing conditions recommended. Flow cytometry: Intracellular staining; recommended dilution: 1-9 μg/ml; positive control: Jurkat cells treated with pervanadate, T-cells from lymph nodes of OT-1 mouse treated with pervanadate.

Key Properties

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Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG2b
Clone No.EM-26
ImmunogenA phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH
TargetCD3 zeta (pY72)
PurificationPurified by protein-A affinity chromatography.
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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CD3 zeta chain, CD247, T3Z, TCRz, IMD25
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
ICC
Immunocytochemistry
View Protocol

Available Sizes

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0.1 mg
$ 280.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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