PRKCG (phospho-T514) antibody

• Catalog Number: orb515529
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PRKCG (phospho-T514)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Primate, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T514 of human PKC gamma protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PRKCG
• Entrez: 5582, 24681
• UniProt ID: P63319, P05129
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: PKCG; Protein kinase C gamma type; PKC-gamma
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PKC gamma (Phospho-T514) expression in rat brain (A) whole cell lysates. (Predicted band size: 78 kD; Observed band size: 80 kD)

Immunohistochemical analysis of PKC gamma (Phospho-T514) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Direct ELISA antibody dose-response curve using Anti-PKC gamma (Phospho-T514) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.