PRKAR2A antibody

• Catalog Number: orb414536
• Category: Antibodies
• Description: Mouse monoclonal antibody to PRKAR2A
• Clonality: Monoclonal
• Clone Number: Hs-36
• Tested applications: ICC, WB
• Reactivity: Human
• Isotype: Mouse IgM
• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• Concentration: 1 mg/ml
• Dilution range: Immunocytochemistry: Recommended dilution: 10 μg/ml; membrane permeabilization (acetone) is essential.
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: PRKAR2A
• Entrez: 5576
• UniProt ID: P13861
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: Anti-PKAR2A antibody, anti-PKR2 antibody, anti-PKA
• Note: For research use only
• Application notes: Immunocytochemistry: Recommended dilution: 10 μg/ml; membrane permeabilization (acetone) is essential.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry staining of normal human sperma with anti-PRKAR2A antibody (intracellular signal in acrosomes, green); DNA visualized by DAPI (blue).