PRKAA1/PRKAA2 Antibody

• Catalog Number: orb1260776
• Category: Antibodies
• Description: PRKAA1/PRKAA2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein of human PRKAA1/PRKAA2
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 64kDa
• Target: PRKAA1/PRKAA2
• UniProt ID: Q13131, P54646
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: AMPK, AMPKa1, FLK1, VEGFR2, AAPK1, AMPK alpha-1 ch
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of A-549 cells, using PRKAA1/PRKAA2 antibody (orb1260776). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of HeLa cells using AMPKa1/AMPKa2 Polyclonal Antibody (orb1260776) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.