PRDX2 Antibody

• Catalog Number: orb1264506
• Category: Antibodies
• Description: PRDX2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Bovine, Hamster, Monkey, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This PRDX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-198 amino acids from the C-terminal region of human PRDX2.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 22 kDa
• Target: PRDX2
• UniProt ID: P32119
• NCBI: P32119
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Peroxiredoxin-2, Natural killer cell-enhancing fac
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from mouse heart and rat heart tissue lysate (from left to right), using PRDX2 Antibody at 1:1000 at each lane.

Fluorescent confocal image of Hela cell stained with PRDX2 Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PRDX2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).PRDX2 immunoreactivity is localized to Cytoplasm significantly.

Western blot analysis of PRDX2 Antibody in MDA-MB231, 293 cell line and mouse brain tissue lysates (35 ug/lane) (2ug/ml)

Formalin-fixed and paraffin-embedded human brain tissue reacted with PRDX2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.