PRDM1 Antibody / BLIMP1

• Catalog Number: orb1823082
• Category: Antibodies
• Description: The development and differentiation of plasma cells, which are terminally differentiated B-cells, are induced by Blimp-1 (B lymphocyte-induced maturation protein, also designated PRDI-BF1). Blimp-1 is a transcriptional repressor that localizes to the nucleus and is considered a master regulator of terminal B-cell development. Alone, Blimp-1 is sufficient to trigger terminal B-cell differentiation. Blimp-1 upregulates the expression of syndecan-1 and J chain, represses IFN-b gene transcription and associates with HDAC to recruit it to DNA, thereby repressing c-Myc. Blimp-1 is expressed during the late stages of B-cell differentiation in immunoglobulin-secreting plasma cells, as well as in long-lived, bone marrow plasma cells. The expression of Blimp-1 defines a checkpoint beyond which fully activated B cells proceed to the plasma cell stage, whereas immature and partially activated cells are eliminated.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-PRDM1-2B9
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Recombinant full-length human protein was used as the immunogen for the BLIMP-1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This BLIMP-1 antibody is available for research use only.
• UniProt ID: O75626
• Storage: Aliquot the BLIMP-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells with PRDM1 antibody (green, clone PCRP-PRDM1-2B9) and CF640R phalloidin (red).

Flow cytometry testing of PFA-fixed human HeLa cells with PRDM1 antibody (clone PCRP-PRDM1-2B9) followed by goat anti-mouse IgG-CF488 (blue); Red = unstained cells.

SDS-PAGE analysis of purified, BSA-free PRDM1 antibody (clone PCRP-PRDM1-2B9) as confirmation of integrity and purity.

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using PRDM1 antibody (clone PCRP-PRDM1-2B9). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.