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Catalog Number | orb767817 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Pragmin (phospho-Tyr413) |
Clonality | Polyclonal |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Pragmin (phospho Tyr413) |
Concentration | 1 mg/ml |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
Conjugation | Unconjugated |
MW | 175 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-SGK223 antibody, anti-Tyrosine-protein kinase Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot analysis of A549 cells using Phospho-Pragmin (Y413) Polyclonal Antibody.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Pragmin (Phospho-Tyr413) Antibody.
Western blot analysis of lysate from A549 cells, using phospho-Pragmin (Phospho-Tyr413) antibody.
Immunohistochemical analysis of paraffin-embedded human spleen tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200.