PR38A Antibody (C-term)

• Catalog Number: orb1927640
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB25755
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse, Other
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 37477 Da
• Target: This PR38A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-312 amino acids from the C-terminal region of human PR38A.
• UniProt ID: Q8NAV1
• NCBI: NP_116253.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Pre-mRNA-splicing factor 38A, PRPF38A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

PR38A Antibody (C-term) western blot analysis in MDA-MB231, CEM, HL-60 cell line lysates (35 ug/lane).This demonstrates the PR38A antibody detected the PR38A protein (arrow).

PR38A Antibody (C-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-PR38A Antibody (C-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 37 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

PR38A Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PR38A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.