PPP4C antibody

• Catalog Number: orb20241
• Category: Antibodies
• Description: Goat polyclonal antibody to PPP4C
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Rat
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 2-4µg/ml
• Conjugation: Unconjugated
• MW: 35.1
• Target: PPP4C
• Entrez: 5531
• Protein Sequence: PQETRGIPSKKP
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PPP4C antibody, anti protein phosphatase 4 (f
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Prostate shows nuclear staining of secretory cells in the gland. Recommended concentration, 2-4ug/ml.WB: Approx 35kDa band observed in HEK293, A431 and HeLa lysates (calculated MW of 35.1kDa according to NP_002711.1). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20241 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb20241 (2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western blot analysis of staining of Rat Spleen (A) and Lung (B) lysate using PPP4C antibody.