PPP2R5C Antibody

• Catalog Number: orb1524130
• Category: Antibodies
• Description: PPP2R5C Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 490 and 524
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PPP2R5C
• UniProt ID: Q13362
• NCBI: NP_002710.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: PR61G; serine/threonine-protein phosphatase 2A 56
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PPP2R5C by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (H; 50 µg) and Jurkat (J; 50 µg) cells. Antibody: Affinity purified rabbit anti-PPP2R5C antibody orb1524130 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human PPP2R5C by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PPP2R5C antibody orb1524130 used for IP at 6 µg/mg lysate.

Detection of human PPP2R5C by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PPP2R5C (Cat. No. orb1524130) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).