PPP1CC Antibody

• Catalog Number: orb1274456
• Category: Antibodies
• Description: PPP1CC Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human PPP1CC (NP_002701.1).
• Concentration: batch dependent
• Dilution range: WB: 1:200 - 1:2000IHC: 1:50 - 1:200CHIP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 38kDa
• Target: PPP1CC
• UniProt ID: P36873
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Serine/threonine-protein phosphatase PP1-gamma cat
• Note: For research use only
• Application notes: WB: 1:200 - 1:2000IHC: 1:50 - 1:200CHIP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry of paraffin-embedded human gastric cancer using PPP1CC antibody (orb1274456) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using PPP1CC antibody (orb1274456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Western blot analysis of extracts from normal (control) and PPP1CC knockout (KO) HeLa cells, using PPP1CC antibody (orb1274456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat brain using PPP1CC antibody (orb1274456) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using PPP1CC antibody (orb1274456) at dilution of 1:100 (40x lens).