PPM1A monoclonal antibody (M03), clone 4E11

• Catalog Number: orb2292362
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full-length recombinant PPM1A.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4E11
• Tested applications: ELISA, IF, IHC-P, PLA, WB
• Reactivity: Human
• Isotype: IgG2a Kappa
• Immunogen: PPM1A (AAH26691, 1 a.a. ~ 382 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPNAPKVSPEAVKKEAELDKYLECRVEEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW
• NCBI: AAH26691
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged PPM1A is 3 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to PPM1A on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to PPM1A on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]

Proximity Ligation Analysis of protein-protein interactions between AKT1 and PPM1A. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-PPM1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Western Blot detection against Immunogen (67.76 KDa).