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Catalog Number | orb527034 |
---|---|
Category | Antibodies |
Description | PPCS Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, IHC-Fr, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence of human PPCS (VEIEEKIVDNLQSRHTAFIGDRN). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 35 kDa |
UniProt ID | Q9HAB8 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Phosphopantothenate--cysteine ligase, Phosphopanto Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of A431 cells using anti-PPCS antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of PPCS using anti-PPCS antibody.Lane 1:human HeLa cell;2:human placenta tissue; 3:human PC-3 cell.
IF analysis of PPCS using anti-PPCS antibody.PPCS was detected in immunocytochemical section of A549 cell.
IF analysis of PPCS using anti-PPCS antibody.PPCS was detected in immunocytochemical section of A431 cell.
IHC analysis of PPCS using anti-PPCS antibody.PPCS was detected in paraffin-embedded section of human intestinal cancer tissue.
IHC analysis of PPCS using anti-PPCS antibody.PPCS was detected in paraffin-embedded section of mouse small intestine tissues.
IHC analysis of PPCS using anti-PPCS antibody.PPCS was detected in paraffin-embedded section of rat small intestine tissues.
FC, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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