PPAR gamma Mouse Monoclonal Antibody

• Catalog Number: orb526703
• Category: Antibodies
• Description: PPAR gamma Mouse Monoclonal Antibody
• Target: PPARG
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human PPAR gamma
• UniProt ID: P37231
• MW: 57 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 4C1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GLM1; CIMT1; NR1C3; PPARG; PPARG1; PPARG2; PPARG5;
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PPAR gamma) Monoclonal Antibody, Unconjugated (orb526703) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Sample: Eye (Mouse) Lysate at 40 ug, Primary: Anti-PPAR gamma (orb526703) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.

Sample: Lane 1: Liver (Mouse) Tissue Lysate at 40 ug, Lane 2: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-PPAR gamma (orb526703) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 50 kD.