PPAR gamma Mouse Monoclonal Antibody

SKU: orb526703

Description

PPAR gamma Mouse Monoclonal Antibody

Research Area

Adipogenesis, Cardiovascular Research, Cell Biology, DNA and RNA Research, Diabetes Research, Fatty Acid Oxidation, Fatty Acids, Metabolism, Obesity, Zinc Finger Protein, cell Biology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 4C1
• Immunogen: KLH conjugated synthetic peptide derived from human PPAR gamma
• Target: PPARG
• Molecular Weight: 57 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GLM1; CIMT1; NR1C3; PPARG; PPARG1; PPARG2; PPARG5; PPARgamma; Nuclear receptor subfamily 1 group C member 3; PAX8/PPARG Fusion Gene; Peroxisome Proliferator Activated Receptor gamma; Peroxisome proliferator activated nuclear receptor gamma variant 1; Peroxisome proliferator activated receptor gamma 1; Peroxisome Proliferator Activated Receptor gamma; Peroxisome proliferator-activated receptor gamma; PPAR gamma; PPAR-gamma; PPARG_HUMAN; PPAR-γ; PPAR γ; PPARγ;

Paraformaldehyde-fixed, paraffin embedded (Mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PPAR gamma) Monoclonal Antibody, Unconjugated (orb526703) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Sample: Eye (Mouse) Lysate at 40 ug, Primary: Anti-PPAR gamma (orb526703) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.

Sample: Lane 1: Liver (Mouse) Tissue Lysate at 40 ug, Lane 2: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-PPAR gamma (orb526703) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 50 kD.

Quick Database Links

Gene Symbol

PPARG

UniProt

P37231