PPAR-gamma Antibody

• Catalog Number: orb1522961
• Category: Antibodies
• Description: PPAR-gamma Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 50 and 100
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PPAR-gamma
• UniProt ID: P37231
• NCBI: NP_056953.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: PPARG1; PPAR-gamma; PPARG2; peroxisome proliferato
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PPAR-gamma by western blot. Samples: Whole cell lysate (50 µg) from U937 cells incubated with (+) or without (-) TPA (200nM, 72 hrs) and lysed using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPAR-gamma antibody orb1522961 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of mouse PPAR-gamma by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, differentiated mouse NIH 3T3-L1, and mouse NIH3T3-L1 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPAR-gamma antibody orb1522961 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of mouse PPAR-gamma by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from differentiated mouse NIH 3T3-L1 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPAR-gamma antibody orb1522961 used for IP at 6 µg per reaction.