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Catalog Number | orb1294232 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to PPARγ |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide |
Dilution range | Western Blot 1:500 – 1:1500 Immunofluorescence 1:300 – 1: |
Conjugation | Unconjugated |
Target | PPARγ |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-PPARγ antibody at 1/1000 dilution, Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with orb1294232 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PPARγ with orb1294232 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PPARγ with orb1294232 at 1/100.
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PPARγ with orb1294232 at 1/100.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294232 anti-PPARγantibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling PPARγ with orb1294232 at 1/100.
ICC, IHC-P, WB | |
Bovine, Gallus, Porcine, Rabbit, Rat, Sheep |
FC, ICC, IHC-P, WB | |
Canine, Guinea pig, Porcine, Rabbit |
FC, ICC, IHC-P, WB | |
Bovine, Gallus, Porcine, Rabbit, Sheep |
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