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Catalog Number | orb667780 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to PP5 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Recombinant fusion protein of human PP5. The exact sequence is proprietary. |
Dilution range | WB: 1-500-2000, IF: 1-50-200 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | PPP5C |
Entrez | 19060, 65179, 5536 |
UniProt ID | Q60676, P53042, P53041 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | Anti-PPP5 antibody, anti-Serine/threonine-protein Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of PP5 expression in HeLa (A), mouse kidney (B), mouse brain (C), rat testis (D) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 57 kD)
Immunofluorescent analysis of PP5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
FC, ICC, IHC, IHC-Fr, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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