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Catalog Number | orb769585 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to PP2A-Calpha (phospho-Tyr307) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human PP2A-alpha around the phosphorylation site of Tyr307. AA range:260-309 |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
MW | 35 |
Storage | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-PPP2CA antibody, anti-Serine/threonine-protei Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of Hela cell. 1, PP2A-Cα (phospho Tyr307) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HSP70 Monoclonal Antibody (3G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
Western blot analysis of SKOV3 using p-PP2A-Cα (Y307) antibody. Antibody was diluted at 1:1000.
Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.
Immunofluorescence analysis of A549 cells, using PP2A-alpha (Phospho-Tyr307) Antibody. The picture on the right is blocked with the phospho peptide.
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