PP2A-alpha/PPP2CA Antibody

• Catalog Number: orb251530
• Category: Antibodies
• Description: PP2A-alpha/PPP2CA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Gallus
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human PP2A-alpha recombinant protein (Position: M1-L309). Human PP2A-alpha shares 100% amino acid (aa) sequence identity with both mouse and rat PP2A-alpha.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 35594 MW
• UniProt ID: P67775
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Serine/threonine-protein phosphatase 2A catalytic
• Note: For research use only
• Application notes: WB: The detection limit for PP2A-alpha is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of K562 cells using anti-PPP2CA antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-PPP2CA antibody.Lane 1:Jurkat cell;2:MCF-7 cell;3:human 293T cell;4:human HeLa cell;5:human HepG2 cell;6:rat brain tissue;7:rat PC-12 cell;8:mouse lung tissue;9:mouse liver tissue;10:mouse brain tissue;11:mouse NIH/3T3 cell.

IF analysis of PPP2CA using anti-PPP2CA antibody. PPP2CA was detected in an immunocytochemical section of U2OS cells.

IHC analysis of PPP2CA using anti-PPP2CA antibody. PPP2CA was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue.

IHC analysis of PPP2CA using anti-PPP2CA antibody. PPP2CA was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue.

IHC analysis of PPP2CA using anti-PPP2CA antibody. PPP2CA was detected in a paraffin-embedded section of human thyroid cancer tissue.

IHC analysis of PPP2CA using anti-PPP2CA antibody. PPP2CA was detected in a paraffin-embedded section of mouse brain tissue.

IHC analysis of PPP2CA using anti-PPP2CA antibody. PPP2CA was detected in a paraffin-embedded section of rat brain tissue.