POU3F3 antibody

• Catalog Number: orb20546
• Category: Antibodies
• Description: Goat polyclonal antibody to POU3F3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:4000, WB: 1-3 μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Conjugation: Unconjugated
• MW: 50.3
• Target: POU3F3 / BRN1 / OCT8
• Entrez: 5455
• Protein Sequence: HMLSHAHQWVTAL
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti Brain-1 antibody, anti BRN1 antibody, anti OT
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 48kDa band observed in Mouse and Rat Spinal Cord lysates (calculated MW of 50.3kDa according to Human NP_006227.1 and 50.2kDa according to Mouse NP_032926.2). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of paraformaldehyde fixed Neuro-2a cells using POU3F3 antibody.

Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells using POU3F3 antibody.

Western blot analysis of Mouse Spinal Cord (A) and Brain (B) lysate using POU3F3 antibody.