POU3F2 Antibody

• Catalog Number: orb1249116
• Category: Antibodies
• Description: POU3F2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Porcine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-AQSLVQGDYGALQSN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 45kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 46.9kDa according to Human NP_005595.2 and 47.1kDa according to Mouse NP_032925.1). Recommended concentration: 0.1-0.3ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: POU3F2
• UniProt ID: P20265
• NCBI: NP_005595.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: POU3F2, POU class 3 homeobox 2, BRN2, OCT7, OTF7,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 45kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 46.9kDa according to Human NP_005595.2 and 47.1kDa according to Mouse NP_032925.1). Recommended concentration: 0.1-0.3ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Expiration Date: 12 months from date of receipt.

orb1249116 (0.1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.