POU2F1 Antibody

• Catalog Number: orb1249118
• Category: Antibodies
• Description: POU2F1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-NVQSKSNEESGDSQQ
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 100kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 76.5kDa according to NP_002688.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: POU2F1
• UniProt ID: P14859
• NCBI: NP_002688.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: POU2F1, POU class 2 homeobox 1, OCT1, OTF1, OTTHUM
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 100kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 76.5kDa according to NP_002688.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249118 (0.5 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.