POU2AF1 antibody

• Catalog Number: orb22486
• Category: Antibodies
• Description: Goat polyclonal antibody to POU2AF1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Canine, Human
• Dilution range: ELISA: 1:8000, WB: 1-2 μg/ml, IHC-P: 3-5µg/ml
• Conjugation: Unconjugated
• MW: 27.4
• Target: POU2AF1
• Entrez: 5450
• Protein Sequence: DSDAYALNHTLSVE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti BOB1 antibody, anti OBF1 antibody, anti OBF-1
• Note: For research use only
• Application notes: IHC: In paraffin embedded Human Spleen shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells. Recommended concentration, 3-5μg/ml.WB: In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HEK293 lysate (10ug protein in RIPA buffer) DYKDDDDK tag in (Lane A), Mock-transfected HEK293 in (Lane B) and anti-DYKDDDDK Tag (1/3000) in (lane C) using POU2AF1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human Spleen shows nuclear tissue using POU2AF1 antibody