POU2AF1 Antibody / BOB-1

• Catalog Number: orb606642
• Category: Antibodies
• Description: BOB1, also called POU2AF1, expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB1. Conversely, only some cases of classical Hodgkin lymphoma show BOB1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BOB1/2423
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 148-255 from the human protein was used as the immunogen for the BOB1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This BOB1 antibody is available for research use only.
• UniProt ID: Q16633
• Storage: Store the BOB1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the BOB1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.