POU1F1 Antibody / PIT-1 / GHF-1

• Catalog Number: orb1716178
• Category: Antibodies
• Description: Transcriptional regulators play a critical role in development by mediating tissue- and cell-specific transcription. POU domain factors are transcriptional regulators characterized by a bipartite DNA binding domain, which consists of two highly conserved regions, tethered by a variable linker of 14-26 amino acids. Pit-1, also known as growth hormone factor-1 (GHF-1), a member of the POU homeodomain family, is essential for the normal development of the anterior pituitary gland, where it is required for the formation of somatotropes, lactotropes and thyrotropes. In somatotropes and lactotropes, Pit-1 activates the production of growth hormone and Prolactin, respectively. In addition, Pit-1 acts as a repressor of gene expression, which allows for the differentiation of specific cell types. Pit-1 is expressed as two alternatively spliced products, designated Pit-1a and Pit-1b, which differ in their trans-activation ability. Mutations in the Pit-1 gene are believed to result in combined pituitary hormone deficiency (CPHD) for growth hormone, Prolactin and thyroid stimulating hormone. The gene which encodes Pit-1 maps to human chromosome 3p11.2.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PIT1/7262
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant human full-length protein was used as the immunogen for the POU1F1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This POU1F1 antibody is available for research use only.
• UniProt ID: P28069
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the POU1F1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human pituitary tissue with POU1F1 antibody (clone PIT1/7262). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.